A. Faraji; B. Esmailpoor; F. Sefidkon; B. Abaszadeh; K. Khavazy
Abstract
In order to investigate the effect of salicylic acid and putrescine as foliar spray on plant yield and growth, essential oil yield and composition of summer Savory (Satureja hortensis L.), a field experiment was carried out based on randomized complete blocks design (RCBD), with three replication. ...
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In order to investigate the effect of salicylic acid and putrescine as foliar spray on plant yield and growth, essential oil yield and composition of summer Savory (Satureja hortensis L.), a field experiment was carried out based on randomized complete blocks design (RCBD), with three replication. Different concentrations of salicylic acid (0, 1, 2 and 3mM) and putrescine (0, 0.5, 1, 1.5 and 2mM) were applied as foliar spray at early stages of shooting, blooming and floweing. Results showed that foliar application of salicylic acid and putrescine had significant effect (p<0.01) on some vegetative parameters such as dry weight and yield of total aerial shoots, leaves and inflorescences as well as total chlorophyll concentration. All treatments had a significant effect (p<0.01) onessential oil efficiency, percentage, yield and composition of Satureja hortensis. The highest value of total aerial shoots dry weight (45.63g/p), total aerial shoots yield (5070kg/ha), leaf dry weight (6.39g/p), leaf yield (710kg/ha), inflorescence dry weight (15/65g/p), inflorescence yield (1739kg/ha), and total chlorophyll concentration (1.46mg/l) were observed in 3mM salicylic acid. The essential oil efficiency (2.35kg/ha) and essential oil yield (76.76%) reached a maximum range in 1.5mm putrescine. The concentration of thymol (47.76%) as the main volatile component of summer savory, reached the highest value in 2mM salicylic acid while other components, such as α-pinene, limonene and E-B-ocimene, reached the highest value in 50mg/L putrescine.
E. Karegar Hajiabadi; B. Abbaszadeh; F. Sefidkon; K. Khavazi
Abstract
This research was aimed to investigate the effects of biological fertilizers on seedling survival rate of Thymus pubescens Bioss. The study was conducted in the research field of Alborz Research Station in 2011 using a factorial arrangement in a randomized complete blocks design with three replications. ...
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This research was aimed to investigate the effects of biological fertilizers on seedling survival rate of Thymus pubescens Bioss. The study was conducted in the research field of Alborz Research Station in 2011 using a factorial arrangement in a randomized complete blocks design with three replications. Treatments included seed inoculation (Glomus intraradices, G. mosseae and without inoculation) and seedling inoculation (Glomus intraradices, G. mosseae and without inoculation). Analysis of variance indicated that seed inoculation significantly affected canopy circle, number of tillers, number of lateral branches, plant height, seedling survival rate, seed colonization rate and shoot yield at p≤0.01 and flowering percentage at p≤0.05. According to the mean comparisons, the highest canopy circle, number of tillers, number of lateral branches, plant height, flowering percentage, seedling survival rate, root colonization rate and shoot yield were obtained for seed inoculation with G. intraradices. In addition, the highest number of tillers, number of lateral branches, plant height, root length, number of lateral roots, root colonization rate, essential oil percentage and shoot yield were obtained for seedling inoculation with G. intraradices. Consequently, in terms of essential oil percentage and shoot yield, better results were recorded for seed inoculation as compared to seedling inoculation.
M.` Ghorbanpour; N. Majnon Hossieni; Sh. Rezazadeh; M. Omidi; K. Khavazi; M. Hatami; R. Ghafarzadegan
Abstract
In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application ...
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In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application (0, 75, 150 and 225 kg/ha N as ammonium nitrate in the form of solution, N0-N3) before the commencement of water deficit stress treatment (30, 60 and 90% depletion of water from field capacity, W1-W3). Extracted alkaloids were analyzed by Gas chromatography /mass spectrometery (GC/MS) analysis using a Younglin Acme 6000 GC system equipped with a flame ionization detector (FID) and HP-5MS capillary column (30 m × 0.25 mm, film thickness 0.25 µm). The identification of alkaloids was based on the comparison of their GC retention time and mass spectra data with their standards substances. Results showed that the highest alkaloid content values in root (HYO: 0.281 %DW; SCO: 0.232 % DW) and shoot (HYO: 0.937%DW; SCO: 0.416%DW) were achieved in plants grown under sever water deficit stress (W3) accompanied with nitrogen supply of 225kg/h (N3). The maximum and minimum (20.52 and 8.95mg.plant-1) total alkaloids yield in whole plant were obtained in N2W1 and N3W3 treatments, respectively. The survey results indicated that H. niger in the treatment of moderate water deficit stress (W2) along with 150kg N.h-1 (N2) in addition to having a good amount of content and performance of both alkaloids, it also contained the highest level of SCO, indicating alkaloid quality.
